Xyn10A, a Thermostable Endoxylanase from Acidothermus cellulolyticus 11B-Reference-Cited by-同舟云学术

Xyn10A, a Thermostable Endoxylanase from Acidothermus cellulolyticus 11B

Published:2010-11 Issue:21 Volume:76 Page:7363-7366
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Barabote Ravi D.¹,Parales Juanito V.²,Guo Ying-Yi¹,Labavitch John M.¹,Parales Rebecca E.²,Berry Alison M.¹

Affiliation:

1. Department of Plant Sciences

2. Department of Microbiology, University of California, Davis, California 95616

Abstract

ABSTRACT We cloned and purified the major family 10 xylanase (Xyn10A) from Acidothermus cellulolyticus 11B. Xyn10A was active on oat spelt and birchwood xylans between 60°C and 100°C and between pH 4 and pH 8. The optimal activity was at 90°C and pH 6; specific activity and K m for oat spelt xylan were 350 μmol xylose produced min −1 mg of protein −1 and 0.53 mg ml −1 , respectively. Based on xylan cleavage patterns, Xyn10A is an endoxylanase, and its half-life at 90°C was approximately 1.5 h in the presence of xylan.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01326-10

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