One-Pot Biosynthesis of l -Aspartate from Maleate via an Engineered Strain Containing a Dual-Enzyme System

Abstract

l -Aspartate is currently produced from fumarate by biological methods, and fumarate is synthesized from maleate by chemical methods in industry. We established a biosynthesis method to produce l -aspartate from maleate that is environmentally responsible, convenient, and efficient. Compared to conventional l -aspartate production, no separation and purification of intermediate products is required, which could greatly improve production efficiency and reduce costs. As environmental issues are attracting increasing attention, conventional chemical methods gradually will be replaced by biological methods. Our results lay an important foundation for the industrialization of l -aspartate biosynthesis from maleate.