Detection of a Mixed Infection in a Culture-Negative Brain Abscess by Broad-Spectrum Bacterial 16S rRNA Gene PCR-Reference-Cited by-同舟云学术

Detection of a Mixed Infection in a Culture-Negative Brain Abscess by Broad-Spectrum Bacterial 16S rRNA Gene PCR

Published:2010-06 Issue:6 Volume:48 Page:2250-2252
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Keller Peter M.¹,Rampini Silvana K.¹²,Bloemberg Guido V.¹

Affiliation:

1. Institute of Medical Microbiology

2. Division and Clinic of Internal Medicine, Department of Internal Medicine, University Hospital Zurich, University of Zurich, Zurich, Switzerland

Abstract

ABSTRACT We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01922-09

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