Separation of Methanotrophic Bacteria by Using Percoll and Its Application to Isolation of Mixed and Pure Cultures-Reference-Cited by-同舟云学术

Separation of Methanotrophic Bacteria by Using Percoll and Its Application to Isolation of Mixed and Pure Cultures

Published:1991-12 Issue:12 Volume:57 Page:3656-3659
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Putzer K. P.¹,Buchholz L. A.¹,Lidstrom M. E.¹,Remsen C. C.¹

Affiliation:

1. Center for Great Lakes Studies and Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin 53201, and Keck Laboratories, 138-78 California Institute of Technology, Pasadena, California 911252

Abstract

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.57.12.3656-3659.1991

Reference16 articles.

1. Anonymous. 1987. Percoll methodology and applications. Density marker beads for calibration of gradients of Percoll p. 1-64. Pharmacia Fine Chemicals Uppsala Sweden.

2. Anthony C. 1982. The biochemistry of methylotrophs p. 3-21. Academic Press Inc. New York.

3. Separation of Syntrophomonas wolfei from Methanospirillum hungatii in syntrophic cocultures by using Percoll gradients;Beaty P. S.;Appl. Environ. Microbiol.,1987

4. A survey of buoyant density of microorganisms in pure cultures and natural samples;Guerrero R.;Microbiologia,1985

5. Isolation and characterization of marine methanotrophs. Antonie van Leeuwenhoek J;Lidstrom M.;Microbiol.,1988

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