Deletion of pgi alters tryptophan biosynthesis in a genetically engineered strain of Escherichia coli-Reference-Cited by-同舟云学术

Deletion of pgi alters tryptophan biosynthesis in a genetically engineered strain of Escherichia coli

Published:1991-10 Issue:10 Volume:57 Page:2995-2999
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Mascarenhas D¹,Ashworth D J¹,Chen C S¹

Affiliation:

1. Western Research Center, Stauffer Chemical Company, Richmond, California 94804.

Abstract

Deletion of the structural gene for phosphoglucose isomerase (pgi) of Escherichia coli dramatically alters the path of glucose catabolism by diverting carbon into the hexose monophosphate shunt. The effect of this genetic alteration on the conversion of glucose to tryptophan by strains optimized for the biosynthesis of this amino acid was determined by using 13C-nuclear magnetic resonance spectroscopy in vivo. Pgi- strains converted glucose to tryptophan almost twice as efficiently as did their Pgi+ counterparts.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.57.10.2995-2999.1991

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