Mediator Engineering of <i>Saccharomyces cerevisiae</i> To Improve Multidimensional Stress Tolerance-Reference-Cited by-同舟云学术

Mediator Engineering of Saccharomyces cerevisiae To Improve Multidimensional Stress Tolerance

Published:2022-04-26 Issue:8 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Qi Yanli¹²^ORCID,Xu Nan³,Li Zehong⁴,Wang Jiaping¹²,Meng Xin¹²,Gao Cong¹²,Chen Jian¹²,Chen Wei¹²,Chen Xiulai¹²,Liu Liming¹²

Affiliation:

1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China

2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China

3. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, China

4. School of Biotechnology, Jiangnan University, Wuxi, China

Abstract

This study identified the role of the KIX domain of Mediator tail subunit Med15 in response to acetic acid, H 2 O 2 , and NaCl in S. cerevisiae . Engineered KIX domain by protein engineering, the mutant strain Med15 V76R/R84K , increased multidimensional stress tolerance and pyruvate production compared with that of S. cerevisiae with its native Med15.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01627-21

Reference73 articles.

1. Altered sterol composition renders yeast thermotolerant

2. Med15B Regulates Acid Stress Response and Tolerance in Candida glabrata by Altering Membrane Lipid Composition

3. Lysine harvesting is an antioxidant strategy and triggers underground polyamine metabolism