Premarket Evaluation of a Commercial Glycoprotein G-Based Enzyme Immunoassay for Herpes Simplex Virus Type-Specific Antibodies

Author:

Ashley Rhoda L.1,Wu Linxian2,Pickering Jerry W.2,Tu Ming-Chieh2,Schnorenberg Laura1

Affiliation:

1. Department of Laboratory Medicine, University of Washington, Seattle, Washington,1and

2. Gull Laboratories, Inc., Salt Lake City, Utah2

Abstract

ABSTRACT A new commercial glycoprotein G-based enzyme immunoassay (gG-EIA) was compared with Western blotting (WB) for detection of herpes simplex virus type 1 (HSV-1) or HSV-2 type-specific antibodies in 193 serum samples. Sensitivity for HSV-1 was 95%; specificity was 96%. Sensitivity for HSV-2 was 98%; specificity was 97%. Twelve of 13 serum samples with equivocal gG-EIA results were serotyped by WB.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference22 articles.

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2. Ashley R. L. Current concepts of laboratory diagnosis of herpes simplex infection Clinical management of herpes viruses. Sacks S. L. Straus S. E. Whitley R. J. Griffiths P. D. 1995 137 171 IOS Press Washington D.C

3. Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera

4. Asymptomatic shedding and subsequent transmission of genital herpes simplex;Barton S. E.;Genitourin. Med.,1987

5. The acquisition of herpes simplex virus during pregnancy;Brown Z. A.;N. Engl. J. Med.,1997

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