Development of an Immunomagnetic Method for Selective Isolation of Actinobacillus pleuropneumoniae Serotype 1 from Tonsils-Reference-Cited by-同舟云学术

Development of an Immunomagnetic Method for Selective Isolation of Actinobacillus pleuropneumoniae Serotype 1 from Tonsils

Published:1998-01 Issue:1 Volume:36 Page:251-254
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gagné Annie¹,Lacouture Sonia¹,Broes André²,D’Allaire Sylvie¹,Gottschalk Marcelo¹

Affiliation:

1. Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6,1and

2. Centre de Développement du Porc du Québec, Sainte-Foy, Québec, Canada G1V 4M42

Abstract

ABSTRACT An immunomagnetic separation technique (IMS) for the selective isolation of Actinobacillus pleuropneumoniae serotype 1 was developed. Superparamagnetic polystyrene beads (immunomagnetic beads [IMBs]) were coated with purified rabbit immunoglobulin G specific for A. pleuropneumoniae serotype 1. The antibody concentration, the number of IMBs, the incubation time, and the temperature of incubation influenced the recovery of the target bacteria. The sensitivity of the IMS technique was 1,000-fold higher than that of direct culture. When tonsils from animals from infected herds were tested, significantly more positive tonsils were detected by the IMS technique (68%) than by the standard procedures (22%). The method represents an innovative and highly sensitive approach for the isolation of A. pleuropneumoniae from carrier animals.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.1.251-254.1998

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