Use of a Gastric Juice-Based PCR Assay To Detect Helicobacter pylori Infection in Culture-Negative Patients-Reference-Cited by-同舟云学术

Use of a Gastric Juice-Based PCR Assay To Detect Helicobacter pylori Infection in Culture-Negative Patients

Published:1998-01 Issue:1 Volume:36 Page:317-320
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Yoshida Haruhiko¹,Hirota Katsutaro²,Shiratori Yasushi¹,Nihei Takeshi²,Amano Shin²,Yoshida Akira³,Kawamata Osamu³,Omata Masao¹

Affiliation:

1. Second Department of Internal Medicine, University of Tokyo, Tokyo 113,1

2. Department of Internal Medicine, Mito Saiseikai Hospital, Ibaraki 311-41,2 and

3. Center for Molecular Biology and Cytogenetics, SRL Inc., Tokyo 192,3 Japan

Abstract

ABSTRACT A gastric juice-based PCR assay was compared with culture, microscopy, and a rapid urease test with specimens from 114 subjects. The PCR and conventional tests were positive for 76 and 62% of the subjects, respectively. The prevalence of gastroduodenal disease and seropositivity for anti- Helicobacter pylori immunoglobulin G were similarly high among conventional-test-positive and PCR-only-positive subjects compared to all-negative ones. The PCR assay is recommended to confirm the H. pylori status of culture-negative peptic-ulcer patients.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.1.317-320.1998

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