Development and Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Based on the 18-Kilodalton Matrix Protein for Diagnosis of Primary EBV Infection

Author:

Chan K. H.1,Luo R. X.2,Chen H. L.3,Ng M. H.2,Seto W. H.1,Peiris J. S. M.2

Affiliation:

1. Department of Microbiology, Queen Mary Hospital,1 and

2. The University of Hong Kong,2 Hong Kong, and

3. Department of Pharmacology and Molecular Science, School of Medicine, John Hopkins University, Baltimore, Maryland3

Abstract

ABSTRACT A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the detection of IgM antibody to the EBV capsid antigen on clinical specimens, the sensitivity and specificity of the new IgM ELISA were 96 and 96%, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Cited by 17 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Epstein-Barr Virus;Manual of Clinical Microbiology;2015-05-26

2. Viral Infections;Henry's Clinical Diagnosis and Management by Laboratory Methods;2011

3. Coinfection with EBV/CMV and other respiratory agents in children with suspected infectious mononucleosis;Virology Journal;2010-09-21

4. No Risk of Maternal EBV Infection for Childhood Leukemia;Cancer Epidemiology Biomarkers & Prevention;2009-09-15

5. EPSTEIN-BARR VIRUS;Feigin and Cherry's Textbook of Pediatric Infectious Diseases;2009

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