Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by Using Blood Dried on Filter Paper

Author:

Fiscus Susan A.1,Brambilla Donald2,Grosso Lynell1,Schock Jody1,Cronin Michael3

Affiliation:

1. Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina1;

2. New England Research Institute, Watertown, Massachusetts2; and

3. Organon Teknika, Durham, North Carolina3

Abstract

ABSTRACT We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity and reproducibility.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

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3. Early detection of human immunodeficiency virus on dried blood spot specimens: sensitivity across serial specimens;Comeau A. M.;J. Pediatr.,1996

4. Prevalence of HIV infection in childbearing women in the United States: surveillance using newborn blood samples;Gwinn M.;JAMA,1991

5. Joint United Nations Programme on HIV/AIDS The HIV/AIDS situation in mid 1996: global and regional highlights. UN AIDS Fact Sheet. July 1. 1996 United Nations New York N.Y

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