Rapid PCR Detection of Helicobacter pylori -Associated Virulence and Resistance Genes Directly from Gastric Biopsy Material-Reference-Cited by-同舟云学术

Rapid PCR Detection of Helicobacter pylori -Associated Virulence and Resistance Genes Directly from Gastric Biopsy Material

Published:1998-12 Issue:12 Volume:36 Page:3689-3690
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Björkholm Britta¹²,Befrits Ragnar¹,Jaup Bernhard³,Engstrand Lars⁴²

Affiliation:

1. Department of Medicine, Karolinska Institute,1 and

2. Department of Clinical Microbiology, University Hospital, Uppsala,2 and

3. Department of Medicine, Lundby Hospital, Göteborg,3Sweden

4. Swedish Institute for Infectious Disease Control,4 Stockholm,

Abstract

ABSTRACT We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.12.3689-3690.1998

Reference19 articles.

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