Affiliation:
1. Department of Microbiology, The George Washington University School of Medicine, Washington, D.C. 20005
Abstract
The identity of a heteropolysaccharide from cell walls of
Mycobacterium tuberculosis
H37Ra with Seibert's tuberculopolysaccharide I was demonstrated by thin-layer chromatography, chemical analysis, and antigenic tests. The polysaccharide of
M. kansasii
was shown to be identical with that of
M. tuberculosis
. Defatted cells were disintegrated by ultrasonic treatment in the presence of glass beads; cell walls were obtained by differential ultracentrifugation. Ethyl alcohol-precipitated carbohydrate extracts were analyzed for protein and nucleic acid; these impurities were removed. Tuberculopolysaccharide I from the mycobacterial culture filtrate is probably derived from a lipopolysaccharide of the cell wall, which is partially removed by chloroform in the intact state. Alkaline extraction releases additional polysaccharide, in varying degrees of association with cell wall murein.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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