Author:
LeChevallier M W,Jakanoski P E,Camper A K,McFeters G A
Abstract
m-T7 agar, designed to improve recoveries of injured total coliforms, was evaluated for its effectiveness as a fecal coliform medium. The time and temperature of preincubation were found to be crucial to the optimal recovery of fetal coliforms. Isolation rates for fecal coliforms on m-T7 agar from sewage effluents were the highest when plates were preincubated at 37 degrees C for 8 h before transfer to 44.5 degrees C for 12 h. The medium was found to produce consistently higher fecal coliform counts than all the other methods tested. Recoveries were 3.1 times greater than the standard m-FC method and 1.7 times greater than the two-layer enrichment, temperature acclimation procedure. Verification rates for fecal coliforms isolated on m-T7 agar averaged 89.0%, whereas verification rates for m-FC agar averaged only 82.8%. Both media isolated similar fecal coliform populations. The advantages of a single medium, highly effective for the isolation of both total and fecal coliforms, are discussed.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference14 articles.
1. American Public Health Association. 1980. Standard methods for the examination of water and wastewater 15th ed. American Public Health Association Washington D.C.
2. Influence of environmental stress on enumeration of indicator bacteria from natural waters;Bissonnette G. K.;AppI. Microbiol.,1975
3. Recovery of Escherichia coli from chlorinated secondary sewage;Braswell J. R.;Appl. Microbiol.,1974
4. Chlorine injury and the enumeration of waterborne coliform bacteria;Camper A. K.;Appl. Environ. Microbiol.,1979
5. Membrane filter method for enumerating Escherichia coli;Dufour A. P.;Appl. Environ. Microbiol.,1981
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献