Phenotypic Detection and Differentiation of Carbapenemase Classes Including OXA-48-Like Enzymes in Enterobacterales and Pseudomonas aeruginosa by a Highly Specialized Micronaut-S Microdilution Assay-Reference-Cited by-同舟云学术

Phenotypic Detection and Differentiation of Carbapenemase Classes Including OXA-48-Like Enzymes in Enterobacterales and Pseudomonas aeruginosa by a Highly Specialized Micronaut-S Microdilution Assay

Published:2020-10-21 Issue:11 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pfennigwerth Niels¹^ORCID,Gatermann Sören G.¹,Körber-Irrgang Barbara²,Hönings Robert²

Affiliation:

1. German National Reference Centre for Multidrug-Resistant Gram-Negative Bacteria, Department of Medical Microbiology, Ruhr-University Bochum, Bochum, Germany

2. MERLIN Gesellschaft für Mikrobiologische Diagnostika mbH, Bornheim-Hersel, Germany

Abstract

The objective of this study was to evaluate the Micronaut-S carbapenemase detection microtiter plate assay for the detection of carbapenemases and Ambler class determination. The Micronaut-S carbapenemase detection microtiter plate was tested using a challenging collection of 154 carbapenemase-producing and 150 carbapenemase-negative clinical strains of Enterobacterales and Pseudomonas aeruginosa . The Micronaut-S carbapenemase detection assay was able to detect 148/154 carbapenemase producers correctly, whereas 5/150 non-carbapenemase-producing isolates tested as false positive.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00171-20

Reference35 articles.

1. Classification of OprD sequence and correlation with antimicrobial activity of carbapenem agents inPseudomonas aeruginosaclinical isolates collected in Japan

2. The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria

3. Of Pseudomonas, porins, pumps and carbapenems

4. Molecular mechanisms of antibiotic resistance

5. Correlation between penicillin-binding protein 2 mutations and carbapenem resistance in Escherichia coli

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