Telomere Variation in Xenopus laevis

Author:

Bassham Susan1,Beam Aaron1,Shampay Janis1

Affiliation:

1. Department of Biology, Reed College, Portland, Oregon 97202

Abstract

ABSTRACT Eukaryotic telomeres are variable at several levels, from the length of the simple sequence telomeric repeat tract in different cell types to the presence or number of telomere-adjacent DNA sequence elements in different strains or individuals. We have investigated the sequence organization of Xenopus laevis telomeres by use of the vertebrate telomeric repeat (TTAGGG) n and blot hybridization analysis. The (TTAGGG) n -hybridizing fragments, which ranged from less than 10 to over 50 kb with frequently cutting enzymes, defined a pattern that was polymorphic between individuals. BAL 31 exonuclease treatment confirmed that these fragments were telomeric. The polymorphic fragments analyzed did not hybridize to 5S RNA sequences, which are telomeric according to in situ hybridization. When telomeric fragments from offspring (whole embryos) were compared to those from the spleens of the parents, the inheritance pattern of some bands was found to be unusual. Furthermore, in one cross, the telomeres of the embryo were shorter than the telomeres of the parents’ spleen, and in another, the male’s testis telomeres were shorter than those of the male’s spleen. Our data are consistent with a model for chromosome behavior that involves a significant amount of DNA rearrangement at telomeres and suggest that length regulation of Xenopus telomeres is different from that observed for Mus spretus and human telomeres.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Reference47 articles.

1. Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology. 1994 John Wiley and Sons Inc. New York N.Y

2. Preparation, restriction, and hybridization analysis of mammalian genomic DNA for pulsed field gel electrophoresis;Barlow D. P.;Methods Mol. Biol.,1992

3. Telomeres: no end in sight;Blackburn E. H.;Cell,1994

4. Telomerase activity in normal and malignant hematopoietic cells;Broccoli D.;Proc. Natl. Acad. Sci. USA,1995

5. Telomeric arrays display high levels of heritable polymorphism among closely related plant varieties;Broun P.;Proc. Natl. Acad. Sci. USA,1992

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