Affiliation:
1. Department of Genetics, University of Georgia, Athens, Georgia 30602-7223
Abstract
ABSTRACT
We have identified a gene in
Escherichia coli
that is required for both the normal decay of mRNA and RNA synthesis. Originally designated
mrsC
(mRNA stability), the
mrsC505
mutation described here is, in fact, an allele of the
hflB/ftsH
locus (R.-F. Wang et al., J. Bacteriol. 180:1929–1938, 1998). Strains carrying the thermosensitive
mrsC505
allele stopped growing soon after the temperature was shifted to 44°C but remained viable for several hours. Net RNA synthesis stopped within 20 min after the shift, while DNA and protein synthesis continued for over 60 min. At 44°C, the half-life of total pulse-labeled RNA rose from 2.9 min in a wild-type strain to 5.9 min in the
mrsC505
single mutant. In an
rne-1 mrsC505
double mutant, the average half-life was 19.8 min. Inactivating
mrsC
significantly increased the half-lives of the
trxA
,
cat
,
secG
, and
kan
mRNAs, particularly in an
mrsC505 pnp-7 rnb-500 rne-1
multiple mutant. In addition, Northern analysis showed dramatic stabilizations of full-length mRNAs in a variety of
mrsC505
multiple mutants at 44°C. These results suggest that MrsC, directly or indirectly, controls endonucleolytic processing of mRNAs that may be independent of the RNase E-PNPase-RhlB multiprotein complex.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献