Practical Guidance for Clinical Microbiology Laboratories: Laboratory Diagnosis of Parasites from the Gastrointestinal Tract

Author:

Garcia Lynne S.1,Arrowood Michael2,Kokoskin Evelyne34,Paltridge Graeme P.5,Pillai Dylan R.6,Procop Gary W.78,Ryan Norbert9,Shimizu Robyn Y.10,Visvesvara Govinda2

Affiliation:

1. LSG & Associates, Santa Monica, California, USA

2. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Division of Foodborne, Waterborne, and Environmental Diseases, Waterborne Disease Prevention Branch, Atlanta, Georgia, USA

3. Public Health Ontario, Ottawa, Ontario, Canada

4. Public Health Laboratories—Ottawa, Ottawa, Ontario, Canada

5. Canterbury Health Laboratories, Christchurch, New Zealand

6. Calgary Laboratory Services, Diagnostic and Scientific Centre, Calgary, Alberta, Canada

7. Enterprise Test Utilization and Pathology Consultative Services, Cleveland, Ohio, USA

8. Molecular Microbiology, Parasitology, and Mycology Laboratories, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio, USA

9. Bacteriology and Parasitology, Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute, Melbourne, Victoria, Australia

10. UCLA Health System, Los Angeles, California, USA

Abstract

SUMMARY This Practical Guidance for Clinical Microbiology document on the laboratory diagnosis of parasites from the gastrointestinal tract provides practical information for the recovery and identification of relevant human parasites. The document is based on a comprehensive literature review and expert consensus on relevant diagnostic methods. However, it does not include didactic information on human parasite life cycles, organism morphology, clinical disease, pathogenesis, treatment, or epidemiology and prevention. As greater emphasis is placed on neglected tropical diseases, it becomes highly probable that patients with gastrointestinal parasitic infections will become more widely recognized in areas where parasites are endemic and not endemic. Generally, these methods are nonautomated and require extensive bench experience for accurate performance and interpretation.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology

Reference283 articles.

1. US Food and Drug Administration Center for Medical Services Centers for Disease Control and Prevention. 1988. Clinical laboratory improvement amendments (CLIA). FDA Washington DC. https://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm124105.htm.

2. Clinical Microbiology Procedures Handbook

3. Garcia LS. 2004. Parasitology, p 9.0.1–9.10.8.3. In Isenberg HD (ed), Clinical microbiology procedures handbook, 2nd ed, vol 2. ASM Press, Washington, DC.

4. Isenberg HD (ed). 1995. Essential procedures for clinical microbiology. ASM Press, Washington, DC.

5. Garcia LS. 2016. Diagnostic medical parasitology, 6th ed. ASM Press, Washington, DC.

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