Hemolysis-in-gel and neutralization tests for determination of antibodies to mumps virus

Author:

Grillner L,Blomberg J

Abstract

A hemolysis-in-gel test for the demonstration of antibodies to mumps virus is described. The results were compared with those of neutralization tests using a modified microtechnique. In the neutralization test viral replication was demonstrated by the hemadsorption of guinea pig erythrocytes, the visibility of which could be further enhanced by the use of o-tolidine. Good correlation was found between the results of the two techniques. The hemolysis-in-gel test was simple to perform, rapid, sensitive, and shown to be a useful test for the demonstration of mumps antibodies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

1. Comparison of neutralization and hemagglutinationinhibition techniques for measuring mumps antibody;Buynak E. B.;Proc. Soc. Exp. Biol. Med.,1967

2. Center for Disease Control. 1972. Hemadsorption neutralization test for the serodiagnosis of mumps p. 465-470. In T. B. Conrath (ed.) Handbook of microtiter procedures Dynatech Co. Cambridge Mass.

3. Factors influencing the agglutinability of red cells. II. The agglutination of bovine red cells previously classified as inagglutinable by the building up of an antibody: globulin lattice on the sensitized cells;Coombs R. R. A.;Br. J. Exp. Pathol.,1951

4. Detection of antigens in tissue culture with the aid of mixed hemadsorption;Espmark A.;Acta Pathol. Microbiol. Scand.,1962

5. Quantitative determination ofserum immunoglobulins in antibody agar plates;Fahey J. L.;J. Immunol.,1965

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