Affiliation:
1. Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York 10461.
Abstract
Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv genomic DNA fragment ivg, which conferred a more rapid in vivo growth rate to H37Ra.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference24 articles.
1. Bloom B. R. M. Tuckman G. V. Kalpana and W. R. Jacobs Jr. Unpublished data.
2. A complementation analysis of the restriction and modification of DNA in Escherichia coli;Boyer H.;J. Mol. Biol.,1969
3. Sur quelques proprietes du bacille tuberculeux d'origine bovine, cultive sur la bile de boeuf glycerinee;Calmette A.;C. R. Acad. Sci. Paris.,1909
4. A comparative study of the virulence of Mycobacterium tuberculosis measured in mice and guinea pigs;Collins F. M.;Am. Rev. Respir. Dis.,1969
5. Delayed-type hypersensitivity and cell-mediated immunity in the pathogenesis of tuberculosis;Dannenberg A. M.;Immunol. Today,1991
Cited by
90 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献