Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model-Reference-Cited by-同舟云学术

Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model

Published:2021-04-27 Issue:2 Volume:12 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Amanat Fatima¹²,Strohmeier Shirin¹,Rathnasinghe Raveen¹²³,Schotsaert Michael¹³,Coughlan Lynda¹^ORCID,García-Sastre Adolfo¹³⁴⁵,Krammer Florian¹^ORCID

Affiliation:

1. Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA

2. Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA

3. Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA

4. Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USA

5. The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA

Abstract

A vaccine for SARS-CoV-2 is urgently needed. A better understanding of antigen design and attributes that vaccine candidates need to have to induce protective immunity is of high importance. The data presented here validate the choice of antigens that contain the PP mutations and suggest that deletion of the polybasic cleavage site may lead to a further-optimized design.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Open Philanthropy

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.02648-20

Reference44 articles.

1. A pneumonia outbreak associated with a new coronavirus of probable bat origin

2. A Novel Coronavirus from Patients with Pneumonia in China, 2019