Optimization of an Assay To Determine Colonization Resistance to Clostridioides difficile in Fecal Samples from Healthy Subjects and Those Treated with Antibiotics-Reference-Cited by-同舟云学术

Optimization of an Assay To Determine Colonization Resistance to Clostridioides difficile in Fecal Samples from Healthy Subjects and Those Treated with Antibiotics

Published:2020-12-16 Issue:1 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Harris Hannah C.¹,Best Emma L.²,Normington Charmaine¹,Saint-Lu Nathalie³,Sablier-Gallis Frédérique³,de Gunzburg Jean³^ORCID,Andremont Antoine³,Wilcox Mark H.¹²,Chilton Caroline H.¹^ORCID

Affiliation:

1. Heath Care Associated Infection Research Group, Leeds Institute of Medical Research, Faculty of Medicine and Health, University of Leeds, Leeds, United Kingdom

2. Department of Microbiology, Leeds Teaching Hospitals NHS Trust, The General Infirmary, Leeds, United Kingdom

3. Da Volterra, Paris, France

Abstract

A healthy, intact gut microbiota is often resistant to colonization by gastrointestinal pathogens. During periods of dysbiosis, however, organisms such as Clostridioides difficile can thrive. We describe an optimized in vitro colonization resistance assay for C. difficile in stool (CRACS) and demonstrate the utility of this assay by assessing changes in colonization resistance following antibiotic exposure. Fecal samples were obtained from healthy volunteers ( n = 6) and from healthy subjects receiving 5 days of moxifloxacin ( n = 11) or no antibiotics ( n = 10).

Funder

Da Volterra

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01401-20

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