Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents-Reference-Cited by-同舟云学术

Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents

Published:1997-06 Issue:6 Volume:35 Page:1620-1623
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Beckwith C S¹,Franklin C L¹,Hook R R¹,Besch-Williford C L¹,Riley L K¹

Affiliation:

1. Department of Veterinary Pathobiology, University of Missouri, Columbia 65211, USA. vmkatie@vetmed.missouri.edu

Abstract

A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of rodents, which is especially important for valuable rodents, such as transgenic mice.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.35.6.1620-1623.1997

Reference20 articles.

1. Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene;Battles J. K.;J. Clin. Microbiol.,1995

2. Helicobacter pylori: microbiology of a `slow' bacterial infection;Blaser M. J.;Trends Microbiol.,1993

3. Ordered cosmid library and highresolution physical-genetic map of Helicobacter pylori strain NCTC11638;Bukanov N. O.;Mol. Microbiol.,1994

4. Helicobacter pylori-induced gastritis in the domestic cat;Fox J. G.;Infect. Immun.,1995

5. Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice;Fox J. G.;J. Clin. Microbiol.,1994

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