HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA

Author:

Wittek R,Menna A,Schümperli D,Stoffel S,Müller H K,Wyler R

Abstract

The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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