Author:
Wyde P R,Wilson S Z,Kramer M J,Sun C S,Knight V
Abstract
Small particle aerosols of a hybrid DNA recombinant human alpha interferon, A/D bgl, and a related DNA recombinant leukocyte interferon, A, were generated and delivered to mice for 23.5 h a day for 4 consecutive days. The antiviral activity of these interferons in delivery reservoirs, in the aerosols generated, and in the lungs of test mice was monitored during and after aerosol administration in cytopathic effect inhibition assays, using vesicular stomatitis virus as the indicator virus. In addition, the activity of these interferons in primary mouse embryo cells against influenza A/HK/68 (H3N2) virus was determined. The results obtained indicated that the interferon particles generated in the continuous aerosol therapy system used in these studies remained biologically active and could be readily detected in both aerosol mists and lungs of test mice; levels of exogenous interferon in the lungs equalled or exceeded levels of interferons produced endogenously during experimentally induced influenza virus infection. Titers of the exogenously administered interferons decreased gradually and disappeared from the lungs between 24 and 48 h after cessation of aerosolization. Recombinant human alpha interferon A/D, but not recombinant leukocyte alpha interferon A, significantly inhibited replication of A/HK/68 virus in primary mouse embryo cells in the in vitro studies.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference40 articles.
1. Baron S. 1963. Mechanism of recovery from viral infection p. 39. In K. M. Smith and M. A. Lauffer (ed.) Advances in virus research vol. 10. Academic Press Inc. New York.
2. Baron S. 1977. The interferon system p. 1-10. In S. Baron and F. Diazzani (ed.) The interferon system: a current review to 1978. Texas reports on biology vol. 10. The University of Texas Medical School at Galveston Press Galveston Tex.
3. Standard sampler for assay of airborne microorganisms;Brachman P. S.;Science,1964
4. Interferon and resistance to upper respiratory virus illness;Cate T. R.;Proc. Soc. Exp. Biol. Med.,1969
5. Clinical trials with exogenous interferon: summary of a meeting;Dunnick J. K.;J. Infect. Dis.,1979
Cited by
12 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献