Author:
Barry A L,Coyle M B,Thornsberry C,Gerlach E H,Hawkinson R W
Abstract
Standard Bauer-Kirby disk tests were performed with 85 selected isolates, each tested in triplicate by four different investigators. Each disk test was observed, and zone diameters were measured, under two lighting conditions (transmitted light and reflected light). The two lighting systems produced similar zone measurements (+/-2 mm) with 96% of the tests. When there were greater differences, zones appeared to be larger when observed with reflected light. Interlaboratory reproducibility was much greater when using reflected light rather than transmitted light. We concluded that zone diameters should be measured from the back of the plate while it is resting on, or held 2 to 3 inches [ca. 5.1 to 7.6 cm] above, a black, nonreflecting, flat surface, illuminated by a reflected light source.
Publisher
American Society for Microbiology
Reference3 articles.
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2. National Committee for Clinical Laboratory Standards. 1975. Performance standards for antimicrobial disc susceptilibity tests. National Committee for Clinical Laboratory Standards Villanova Pa.
3. Searle S. R. 1971. Linear models p. 376-420. John Wiley and Sons Inc. New York.
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