Conjugation in Escherichia coli

Author:

Boyer Herbert1

Affiliation:

1. Department of Microbiology, Yale University, New Haven, Connecticut

Abstract

Boyer, Herbert (Yale University, New Haven, Conn.). Conjugation in Escherichia coli . J. Bacteriol. 91: 1767–1772. 1966.—The sex factor of Escherichia coli K-12 was introduced into an E. coli B/r strain by circumventing the host-controlled modification and restriction incompatibilities known to exist between these closely related strains. The sexual properties of the constructed F + B strain and its Hfr derivatives were examined. These studies showed that the E. coli strain B/r F + and Hfr derivatives are similar to the E. coli strain K-12 F + and Hfr derivatives. However, the site of sex factor integration was found to be dependent on the host genome.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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