Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments

Author:

Brim Hassan1,Venkateswaran Amudhan1,Kostandarithes Heather M.2,Fredrickson James K.2,Daly Michael J.1

Affiliation:

1. Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814

2. Pacific Northwest National Laboratory, Richland, Washington 99352

Abstract

ABSTRACT Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans , which is being engineered for in situ bioremediation of radioactive wastes. We report that D . geothermalis is transformable with plasmids designed for D . radiodurans and have generated a Hg(II)-resistant D . geothermalis strain capable of reducing Hg(II) at elevated temperatures and in the presence of 50 Gy/h. Additionally, D . geothermalis is capable of reducing Fe(III)-nitrilotriacetic acid, U(VI), and Cr(VI). These characteristics support the prospective development of this thermophilic radiophile for bioremediation of radioactive mixed waste environments with temperatures as high as 55°C.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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