Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28

Author:

Ferretti J J,Gilpin M L,Russell R R

Abstract

The complete nucleotide sequence was determined for the Streptococcus sobrinus MFe28 gtfI gene, which encodes a glucosyltransferase that produces an insoluble glucan product. A single open reading frame encodes a mature glucosyltransferase protein of 1,559 amino acids (Mr, 172,983) and a signal peptide of 38 amino acids. In the C-terminal one-third of the protein there are six repeating units containing 35 amino acids of partial homology and two repeating units containing 48 amino acids of complete homology. The functional role of these repeating units remains to be determined, although truncated forms of glucosyltransferase containing only the first two repeating units of partial homology maintained glucosyltransferase activity and the ability to bind glucan. Regions of homology with alpha-amylase and glycogen phosphorylase were identified in the glucosyltransferase protein and may represent regions involved in functionally similar domains.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference36 articles.

1. Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis;Aoki H.;Infect. Immun.,1986

2. Cloning and expression of a Streptococcus mutans glucosyltransferase gene in Bacillus subtilis;Burne R. A.;Gene,1986

3. Ciardi J. 1983. Purification and properties of glucosyltransferases of Streptococcus mutans: a review p. 51-64. In R. J. Doyle and J. E. Ciardi (ed.) Glucosyltransferases glucans sucrose and dental caries (a special supplement to Chemical Senses). Information Retrieval Limited Washington D.C.

4. Coykendall A. L. and K. B. Gustafson. 1986. Taxonomy of Streptococcus mutans p. 21-28. In S. Hamada S. M. Michalek H. Kiyono L. Menaker and J. R. McGhee (ed.) Molecular microbiology and immunobiology of Streptococcus mutans. Elsevier Science Publishing Inc. New York.

5. Outer membrane of gram-negative bacteria. XII. Molecular-seiving function of cell wall;Decad G. M.;J. Bacteriol.,1976

Cited by 211 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3