Author:
Hitzeman R A,Hanel A M,Price A R
Abstract
Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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