Molecular Method for Discrimination between Francisella tularensis and Francisella -Like Endosymbionts-Reference-Cited by-同舟云学术

Molecular Method for Discrimination between Francisella tularensis and Francisella -Like Endosymbionts

Published:2008-09 Issue:9 Volume:46 Page:3139-3143
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Escudero Raquel¹,Toledo A.¹²,Gil Horacio¹,Kovácsová Katarina¹,Rodríguez-Vargas Manuela¹,Jado Isabel¹,García-Amil Cristina¹,Lobo Bruno¹,Bhide Mangesh¹,Anda Pedro¹

Affiliation:

1. Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain

2. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain

Abstract

ABSTRACT Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella -like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella -like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida -like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00275-08

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