Affiliation:
1. Microbial Ecology Group
2. Genomics Unit, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom
Abstract
ABSTRACT
The bacterium
Clostridium saccharolyticum
K10, isolated from a fecal sample obtained from a healthy donor who had received long-term tetracycline therapy, was found to carry three tetracycline resistance genes:
tet
(W) and the mosaic
tet
(O/32/O), both conferring ribosome protection-type resistance, and a novel, closely linked efflux-type resistance gene designated
tet
(40).
tet
(40) encodes a predicted membrane-associated protein with 42% amino acid identity to
tetA
(P). Tetracycline did not accumulate in
Escherichia coli
cells expressing the Tet(40) efflux protein, and resistance to tetracycline was reduced when cells were incubated with an efflux pump inhibitor.
E. coli
cells carrying
tet
(40) had a 50% inhibitory concentration of tetracycline of 60 μg/ml. Analysis of a transconjugant from a mating between donor strain
C. saccharolyticum
K10 and the recipient human gut commensal bacterium
Roseburia inulinivorans
suggested that
tet
(O/32/O) and
tet
(40) were cotransferred on a mobile element. Sequence analysis of a 37-kb insert identified on the basis of tetracycline resistance from a metagenomic fosmid library again revealed a tandem arrangement of
tet
(O/32/O) and
tet
(40), flanked by regions with homology to parts of the VanG operon previously identified in
Enterococcus faecalis
. At least 10 of the metagenomic inserts that carried
tet
(O/32/O) also carried
tet
(40), suggesting that
tet
(40), although previously undetected, may be an abundant efflux gene.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
42 articles.
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