Rapid diagnosis of cytomegalovirus by indirect immunofluorescence assay with monoclonal antibody F6b in a commercially available kit

Author:

Lucas G1,Seigneurin J M1,Tamalet J1,Michelson S1,Baccard M1,Delagneau J F1,Deletoille P1

Affiliation:

1. Diagnostics Pasteur, Marnes la Coquette, France.

Abstract

Anti-human cytomegalovirus (CMV) monoclonal antibody (MAb) F6b produced by clone 95/12 was used for the rapid diagnosis (16 to 24 h) of CMV isolates in 308 clinical specimens and compared with classic isolation. MAb F6b gave 100% correlation with isolation. When this MAb was used for direct diagnosis with 212 urine and bronchoalveolar specimens, detection of CMV varied from 50 to 75% compared with viral isolation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference28 articles.

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