Characterization of Tn3000, a Transposon Responsible forblaNDM-1Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

Abstract

ABSTRACTInEnterobacteriaceae, theblaNDMgenes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producingEscherichia coliandEnterobacter hormaecheistrains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring theblaNDM-1gene, one from each strain, showed that they belonged to incompatibility groups IncFIIKand IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that theblaNDM-1gene may be transmitted by Tn3000in different parts of the world.