Method for Increasing the Regularity of Isolation of Infectious Ribonucleic Acid from Influenza Virus

Author:

Chetverikova L. K.1,Polyak R. Y.1,Yukhnova L. G.1,Smorodintsev A. A.1

Affiliation:

1. Laboratory of Biochemistry, All-Union Research Institute of Influenza, Ministry of Health, Leningrad USSR

Abstract

A more effective method of isolating infectious ribonucleic acid (RNA) from influenza virus was evaluated based on the enzymatic disintegration of the viral coat by Pronase, followed by phenol-detergent extraction of the RNA from susceptible and from resistant cells.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference25 articles.

1. Isolation of highmolecular weight P-32, labeled influenza virus ribonucleic acid;Agrawal H. O.;Proc. Nat. Acad. Sci. U.S.A.,1966

2. The action of lipids on infectious properties of ribonucleic acid isolated from Ranikhet disease virus;Babbar 0.;Acta Virol.,1968

3. The action of protease on influenza A2 virus;Biddle F.;J. Gen. Virol.,1968

4. Morphological modifications of Semliki Forest virus after treatment by Pronase;Calberg-Bacq C. M.;Acta Virol.,1966

5. Modifications of particles of Newcastle virus disease after treatment by Pronase or Caseinase;Calberg-Bacq C. M.;Acta Virol.,1967

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