Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results

Author:

Pedneault L1,Robillard L1,Turgeon J P1

Affiliation:

1. Department of Microbiology and Immunology, Université de Montréal, Québec, Canada.

Abstract

The Pathfinder respiratory syncytial virus (RSV) enzyme immunoassay (EIA) (Kallestad), the shell vial (SV) technique, and conventional cell culture (CC) were compared for detection of RSV in nasopharyngeal aspirates. We found sensitivities, specificities, and positive and negative predictive values of 58.4, 100, 100, and 68.2%; 80.7, 97.2, 97.0, and 81.9%; and 77.6, 97.2, 96.9, and 79.5% for the CC, EIA, and SV methods, respectively. The SV and EIA techniques were both more sensitive than CC (P < 0.001). Finally, 29 respiratory viruses other than RSV were identified by CC.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference19 articles.

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3. Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay;Bromberg K.;J. Clin. Microbiol.,1987

4. Gleaves C. A. T. F. Smith E. A. Shuster and G. R Pearson. 1984. Rapid detection of cytomegalovirus in MRC-5 cells inoculated 2864 NOTES

5. Hall C. B. 1992. Respiratory syncytial virus p. 1633-1656. In R. Feigin and J. Cherry (ed.) Textbook of pediatric infectious diseases 3rd ed. W. B. Saunders Philadelphia Pa.

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