Detection of Candida albicans and other yeasts in blood by PCR-Reference-Cited by-同舟云学术

Detection of Candida albicans and other yeasts in blood by PCR

Published:1994-01 Issue:1 Volume:32 Page:228-231
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Holmes A R¹,Cannon R D¹,Shepherd M G¹,Jenkinson H F¹

Affiliation:

1. Department of Oral Biology and Oral Pathology, School of Dentistry, University of Otago, Dunedin, New Zealand.

Abstract

Primers complementary to the region of genes coding for rRNA in Candida albicans were used in PCRs to detect yeast DNA extracted from blood samples containing various Candida species. One fragment (105 bp) was amplified from all yeasts tested, whereas a second (684 bp) was only amplified when C. albicans DNA was present. The level of sensitivity was 15 +/- 5 (mean +/- standard error) CFU of C. albicans per ml of blood.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.32.1.228-231.1994

Reference19 articles.

1. Detection of surgical pathogens by in vitro amplification. Part 1. Rapid identification of Candida albicans by in vitro amplification of a fungus-specific gene;Buchman T. G.;Surgery,1990

2. Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae;Cannon R. D.;Mol. Gen. Genet.,1990

3. Cutler J. E. P. M. Glee and H. L. Horn. 1988. Candida albicans- and Candida stellatoidea-specific DNA fragment. J. NOTES 231

4. New centrifugation blood culture device;Dorn G. L.;J. Clin. Microbiol.,1978

5. Invasive candida infections;Edwards J. E.;N. Engl. J. Med.,1991

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