Evaluation of new transport medium for detection of herpes simplex virus by culture and direct enzyme-linked immunosorbent assay

Author:

Ogburn J R1,Hoffpauir J T1,Cole E1,Hood K1,Michael D1,Nguyen T1,Raden S1,Raju B1,Reisinger V1,Oefinger P E1

Affiliation:

1. Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston Medical School 77030.

Abstract

The transport medium Multi-Microbe Media (M4) was evaluated prospectively by culture and direct enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus from 473 specimens. In addition, 377 specimens in Bartels Viral Transport Medium were evaluated. By using culture as a "gold standard," the ELISA sensitivity was approximately 85%, while the specificities exceeded 96% for both media.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

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4. Detection of Chlamydia trachomatis cervical infection by urine tests among adolescents clinics;Journal of Adolescent Health;1998-03

5. An update on the collection and transport of specimens for viral culture;Clinical Microbiology Newsletter;1997-04

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