Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2)-Reference-Cited by-同舟云学术

Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2)

Published:1995-08 Issue:15 Volume:177 Page:4544-4548
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bedford D J¹,Schweizer E¹,Hopwood D A¹,Khosla C¹

Affiliation:

1. Department of Chemical Engineering, Stanford University, California 94305-5025, USA.

Abstract

The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.177.15.4544-4548.1995

Reference41 articles.

1. The nucleotide sequence of the 16S rRNA gene from Streptomyces coelicolor A3(2);Baylis H. A.;Nucleic Acids Res.,1987

2. The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum: its gene structure relative to that of other polyketide synthases;Beck J.;Eur. J. Biochem.,1990

3. Analysis of the nucleotide sequence of the tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis;Bibb M. J.;EMBO J.,1989

4. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea;Cortes J.;Nature (London),1990

5. Crosby J. D. H. Sherman M. J. Bibb W. P. Revill D. A. Hopwood and T. J. Simpson. Polyketide synthase acyl carrier proteins from Streptomyces: expression in Escherichia coli purification and partial characterisation. Biochim. Biophys. Acta in press.

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