Affiliation:
1. Departamento de Microbiología Molecular, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos.
Abstract
We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interrupt one or both pyk genes in the E. coli chromosome. With these constructions, we were able to study the role of these isoenzymes in pyruvate biosynthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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