Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation

Author:

Ang B J,Nickerson K W

Abstract

A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference13 articles.

1. The zonal ultracentrifuge. A new instrument for fractionating mixtures of particles;Anderson N. G.;J. Phys. Chem.,1962

2. Extraction, purification, and properties of Bacillus sotto toxin;Angus T. A.;Can. J. Microbiol.,1956

3. Separation of bacterial spores and parasporal bodies with a fluorocarbon;Angus T. A.;J. Insect Pathol.,1959

4. Cooksey K. E. 1971. The protein crystal toxin of Bacillus thuringiensis: biochemistry and mode of action p. Biological control of insects and mites. Academic Press Inc. London.

5. Immunological homology between crystal and spore protein of Bacillus thuringiensis;Delafield F. P.;J. Bacteriol.,1968

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