Prenatal Diagnosis of Congenital Human Cytomegalovirus Infection in Amniotic Fluid by Nucleic Acid Sequence-Based Amplification Assay

Author:

Revello M. Grazia1,Lilleri Daniele1,Zavattoni Maurizio1,Furione Milena1,Middeldorp Jaap2,Gerna Giuseppe1

Affiliation:

1. Servizio di Virologia, Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, 27100 Pavia, Italy

2. Free University of Amsterdam, Amsterdam, The Netherlands

Abstract

ABSTRACT Nucleic acid sequence-based amplification assays for detection of human cytomegalovirus (HCMV) immediate-early and pp67 mRNA in 65 amniotic fluid samples tested for prenatal diagnosis of congenital HCMV infection showed sensitivity, specificity, and negative and positive predictive values >90%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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