Detection of Single Cells of Cryptococcus neoformans in Clinical Samples by Solid-Phase Cytometry-Reference-Cited by-同舟云学术

Detection of Single Cells of Cryptococcus neoformans in Clinical Samples by Solid-Phase Cytometry

Published:2003-04 Issue:4 Volume:41 Page:1736-1737
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bauters T. G. M.¹,Swinne D.²,Stove V.³,Nelis H. J.¹

Affiliation:

1. Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University

2. Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium

3. Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University Hospital, Ghent

Abstract

ABSTRACT A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.4.1736-1737.2003

Reference6 articles.

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2. Bialek, R., M. Weiss, K. Bekure-Nemariam, L. K. Najvar, M. B. Alberdi, J. R. Graybill, and U. Reischl. 2002. Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays. Clin. Diagn. Lab. Immunol.9:461-469.

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