New PCR Test That Recognizes All Human Prototypes of Enterovirus: Application for Clinical Diagnosis-Reference-Cited by-同舟云学术

New PCR Test That Recognizes All Human Prototypes of Enterovirus: Application for Clinical Diagnosis

Published:2003-04 Issue:4 Volume:41 Page:1750-1752
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bourlet Thomas¹,Caro Valerie²,Minjolle Sophie³,Jusselin Isabelle³,Pozzetto Bruno¹,Crainic Radu²,Colimon Ronald³

Affiliation:

1. Laboratoire de Bactériologie-Virologie, GIMAP, Faculté de Médecine, St-Etienne

2. Laboratoire d'Epidémiologie moléculaire des Entérovirus, Institut Pasteur, Paris

3. Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Rennes, France

Abstract

ABSTRACT We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.4.1750-1752.2003

Reference23 articles.

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