Physical Map of the DNA Genome of Autographa californica Nuclear Polyhedrosis Virus

Abstract

A physical map of the 88 × 10 6 dalton, circular DNA genome of Autographa californica nuclear polyhedrosis virus was constructed. The complete order of Bam HI and Xma I restriction enzyme sites was determined. The Eco RI and Hin dIII fragments were partially ordered, and their general locations, relative to the Bam HI and Xma I maps, were determined. Alterations in the restriction endonuclease fragment patterns of natural genotypic variants of A. californica nuclear polyhedrosis virus, including Trichoplusia ni MEV nuclear polyhedrosis virus, were located on the physical map. Alterations were found throughout the A. californica nuclear polyhedrosis virus DNA genome.