Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using a Synthetic Convergent Peptide Library, or Mixotope, for Diagnosis of Primary EBV Infection-Reference-Cited by-同舟云学术

Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using a Synthetic Convergent Peptide Library, or Mixotope, for Diagnosis of Primary EBV Infection

Published:1999-07 Issue:7 Volume:37 Page:2366-2368
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Tranchand-Bunel D.¹,Gras-Masse H.²,Bourez B.³,Dedecker L.³,Auriault C.¹

Affiliation:

1. Laboratoire d’Immunologie Cellulaire, CNRS UMR 8527,1

2. Laboratoire de Synthèse, Structure et Fonction des Biomolécules, CNRS UMR 8525,2 and

3. Laboratoire d’Immunologie-Sérologie, Département Biologie Spécialisée,3 Institut Pasteur de Lille, 59019 Lille, France

Abstract

ABSTRACT An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.7.2366-2368.1999

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