Molecular Cloning of Cyanobacterial Pteridine Glycosyltransferases That Catalyze the Transfer of either Glucose or Xylose to Tetrahydrobiopterin-Reference-Cited by-同舟云学术

Molecular Cloning of Cyanobacterial Pteridine Glycosyltransferases That Catalyze the Transfer of either Glucose or Xylose to Tetrahydrobiopterin

Published:2010-11-15 Issue:22 Volume:76 Page:7658-7661
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Lee Yeol Gyun¹,Kim Ae Hyun¹,Park Mi Bi¹,Kim Hye-Lim¹,Lee Kon Ho²,Park Young Shik¹

Affiliation:

1. FIRST Research Group, School of Biological Sciences, Inje University, Kimhae 621-749, South Korea

2. Department of Microbiology, School of Medicine, Gyeongsang National University, Jinju 660-751, South Korea

Abstract

ABSTRACT Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherichia coli for an in vitro activity assay.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01083-10

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