Comparisons of Protocols for Decontamination of Environmental Ice Samples for Biological and Molecular Examinations-Reference-Cited by-同舟云学术

Comparisons of Protocols for Decontamination of Environmental Ice Samples for Biological and Molecular Examinations

Published:2004-04 Issue:4 Volume:70 Page:2540-2544
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Rogers S. O.¹²,Theraisnathan V.¹,Ma L. J.²,Zhao Y.²,Zhang G.¹,Shin S.-G.¹,Castello J. D.²,Starmer W. T.³

Affiliation:

1. Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403

2. Faculty of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210

3. Department of Biology, Syracuse University, Syracuse, New York 13244

Abstract

ABSTRACT Drilling and laboratory manipulations of glacial ice cores introduce contemporary microbes and biomolecules onto the cores. We report herein a systematic comparative study of several decontamination protocols. Only treatment with 5% sodium hypochlorite eliminated all external contaminating microbes and nucleic acids while maintaining the integrity of those within the cores.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.4.2540-2544.2004

Reference20 articles.

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