Affiliation:
1. Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany
Abstract
ABSTRACT
The effect of nitrogen regulation on the level of transcriptional control has been investigated in a variety of bacteria, such as
Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli
, and
Streptomyces coelicolor
; however, until now there have been no data for mycobacteria. In this study, we found that the OmpR-type regulator protein GlnR controls nitrogen-dependent transcription regulation in
Mycobacterium smegmatis
. Based on RNA hybridization experiments with a wild-type strain and a corresponding mutant strain, real-time reverse transcription-PCR analyses, and DNA binding studies using cell extract and purified protein, the
glnA
(msmeg_4290) gene, which codes for glutamine synthetase, and the
amtB
(msmeg_2425) and
amt1
(msmeg_6259) genes, which encode ammonium permeases, are controlled by GlnR. Furthermore, since
glnK
(msmeg_2426), encoding a PII-type signal transduction protein, and
glnD
(msmeg_2427), coding for a putative uridylyltransferase, are in an operon together with
amtB
, these genes are part of the GlnR regulon as well. The GlnR protein binds specifically to the corresponding promoter sequences and functions as an activator of transcription when cells are subjected to nitrogen starvation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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