Conjugative plasmids isolated from bacteria in marine environments show various degrees of homology to each other and are not closely related to well-characterized plasmids

Abstract

Mercury resistance plasmids were exogenously isolated, i.e., recovered after transfer to a model recipient bacterium, from marine air-water interface, bulk water, and biofilm communities during incubation in artificial seawater without added nutrients. Ninety-five plasmids from different environments were classified by restriction endonuclease digestion, and 12 different structural plasmid groups were revealed. The plasmid types isolated from different habitats and from different sampling occasions showed little similarity to each other based on their restriction endonuclease patterns, indicating high variation and possibly a low transfer between microhabitats and/or a different composition of the microbial communities at different sites and times. With another approach in which probes derived from one of the isolated plasmids and a mercury resistance (mer) probe from Tn501 were used, similarities between plasmids from several different groups were found. The plasmids were further tested for their incompatibility by use of the collection of inc/rep probes (B/O, com9, FI, FII, HI1, HI2, I1, L/M, N, P, Q, U, W, Y) described by Couturier et al. (M. F. Couturier, P. Bex, L. Bergquist, and W. K. Maas, Microbiol. Rev. 52:375-395, 1988). Hybridizations did not reveal any identity between the 12 plasmid groups and any of the inc/rep probes tested. The results indicate that plasmids isolated from different marine habitats have replication and/or incompatibility systems that are different from the well-characterized plasmids that are commonly used in plasmid biology. This shows the need for the use of more relevant plasmids in studies of plasmid activity in the environment and development of new inc/rep probes for their characterization.